2x bimaketm sybr green master mix component templatec forward primerd 5 µm reverse primerd 5 µm rox reference dye distilled water dh 2o total reaction volumec amount per reaction µl amount per reaction µl variable 2 5 2 5 1 add to 50 50 variable 1 1 0 4 add to 20 20 1 100 ng 0 2 1 µm 0 2 1 µm 1x protocol component 5 ml 200. Sybr green i dye amplitaq fast dna polymerase up ultra pure uracil dna glycosylase udg. Kapa sybr fast qpcr master mix 2x is stable through 30 freeze thaw cycles.
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